Analysis Note

ControlHyperplastic lymph nodes.

Application

Anti-Vimentin Antibody, clone LN-6 detects level of Vimentin & has been published & validated for use in IH(P).

Immunohistochemistry on B5 fixed, paraffin embedded tissue sections.

General description

Vimentin is a class III intermediate filament protein (homopolymers) found in various non-epithelial cells including mesenchymal cells and fibroblasts. In many cells of mesenchymal origin, it is one of the most prominent phosphoproteins, especially during cell division when enhanced phosphorylation of vimentin corresponds with the reorganization of vimentin filaments.

Immunogen

Nuclei from adult human thymic biopsy specimens.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Liquid tissue culture supernatant containing 0.02% sodium azide.

Specificity

Reacts with human mesenchymal cells and derived sarcomas. Reacts with an epitope of the cytoskeletal protein vimentin but unlike other vimentin antibodies, does not react with human lymphoid cells or derived malignancies. Reactive with endothelium, muscle, fibroblasts, melanocytes, peripheral nerve, sertoli cells, kidney mesangial cells and tubules, osteoblasts and periosteum, non-hematopoietic tumors of mesenchymal origin (all sarcomas), meningiomas and melanomas.

Target description

57-60 kDa